Abnova/7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit/1kit/KA1293
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit is a fluorescence-labeled method for determining the cytotoxic effect of immune effector cells at a single cell level.
- Storage Instruction:
- Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.
- Protocol:
Protocol Download
- MSDS:
- Download
- Regulation Status:
- For research use only (RUO)
- Datasheet:
- Download
- Applications
- Flow Cytometry
- The degree of cytotoxicity of natural killer cells (effector cells; NK cells) on K-562 cells (target cells) depends on the ratio of NK cells to K-562 cells. K-562 cells were labeled with CFSE and co-cultured with NK cells at the ratios indicated above. Cells were co-cultured for four hours at 37°C. At the end of the experiment, dead cells were labeled with 7-AAD. The percentage of K-562 cell death was analyzed by a flow cytometer. When K-562 cells were cultured alone, there is little cell death (2%). Addition of NK cells at a ratio as low as 6.25 : 1 caused a significant increase in K-562 cell death (8.2%). At a ratio of NK cells to target cells of 25 : 1, there is 19.7% death of K-562 cells.
- Application Image
- Flow Cytometry
- enlarge
品牌介绍
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