Abnova/2-NBDG葡萄糖摄取测定试剂盒/1kit/KA6077
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美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- 2-NBDG Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay for measuring glucose uptake in cultured cells. The fluorescence signal can be monitored by fluorescence microscope or flow cytometer with a 488 nm laser and 530/30 nm emission filter (FITC channel). This Assay Kit is the most robust tool for monitoring glucose transporters.
- Storage Instruction:
- Store the kit at -20°C and avoid from light.
- Note:
- Example Data Analysis and Figures.
Fluorescence images of 2-NBDG uptake in CHO-K1 cells using the 2-NBDG Glucose Uptake Assay Kit. CHO-K1 cells at 40,000 cells/well/100 uL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with 20 mM Glucose (B) or 100 uM Phloretin (C) at 37°C for 1 hour, then incubated with 100 uM 2-NBDG staining solution for 20 minutes. Untreated control cells were stained under the same conditions. The fluorescence signal was measured using a fluorescence microscope with FITC filter.
- Protocol:
Protocol Download
- Suitable Sample:
- Cultured Cells.
- Detection Method:
- Fluorimetric
- Platform:
- Instrument: Flow cytometerExcitation: 488 nm laserEmission: 530/30 nm filterInstrument specification(s): FITC channelInstrument: Fluorescence microscopeExcitation: FITC filterEmission: FITC filterRecommended plate: Black wall/clear bottom
- Regulatory Status:
- For research use only (RUO)
- Datasheet:
- Download
- Applications
- Functional Study
- Flow Cytometry
- Flow cytometry of 2-NBDG uptake in CHO-K1 cells using the 2-NBDG Glucose Uptake Assay Kit. CHO-K1 cells were treated with or without 100 uM Phloretin at 37°C for 1 hour, then incubated with 100 uM 2-NBDG staining solution for 20 minutes. To prepare adherent CHO-K1 cells for flow cytometry, EDTA was used to detach cells after staining. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
- Application Image
- Functional Study
- Flow Cytometry
- enlarge
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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