Abnova/SARS-CoV-2 N recombinant monoclonal antibody, clone 85E9/1kit/RAB01065
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联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Recombinant rabbit monoclonal antibody raised against SARS-CoV-2 N.
- Immunogen:
- A synthetic peptide corresponding to C-terminus of SARS-CoV-2 N.
- Host:
- Rabbit
- Reactivity:
- SARS-CoV, SARS-CoV-2
- Specificity:
- Highly-sensitive to both SARS-CoV-2 and SARS-CoV nucleoprotein.
- Form:
- Liquid
- Preparation Method:
- 85E9 is derived from a Fab phage display library made from a rabbit immunized with synthetic peptide specific to the C-terminus of SARS-CoV-2 nucleocapsid protein. Libraries were selected on N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and Fab sup were screened on SARS-CoV-2 NP and SARS-CoV (2003) NP by ELISA. Positive clones were cloned into a bi-cistronic IgG vector and produced in HEK293 cells. The antibodies were purified from a Protein A column.
- Purification:
- Protein A purification
- Concentration:
- 0.5 mg/mL
- Isotype:
- IgG1, kappa
- Recommend Usage:
- ELISAThe optimal working dilution should be determined by the end user.
- Storage Buffer:
- In 1X PBS (0.02% sodium azide).
- Storage Instruction:
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Note:
- 75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), 84D7 (Cat # RAB01057), 85C1 (Cat # RAB01059), 85C10 (Cat # RAB01061), 85B4 (Cat # RAB01063), and 85E9 (Cat # RAB01065) can be directly coated to the ELISA wells as a capture antibody and the other antibody (e.g. biotinylated) [75G5a (Cat # RAB01054), 84C4a (Cat # RAB01056), 84D7 (Cat # RAB01058), 85C1 (Cat # RAB01060), 85C10 (Cat # RAB01062), 85B4 (Cat # RAB01064), and 85E9 (Cat # RAB01066)] can be used as a detecting antibody with StreptAvidin-HRP. For highest sensitivity, we recommend pairing unconjugated clone 75G5a (Cat # RAB01053), immobilized on the ELISA plate to capture COVID-19 NP antigen, with SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin) (Cat # RAB01062) for detection using high-sensitivity streptavidin-HRP (Pierce #21130).This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Datasheet:
Download
- Applications
- Western Blot (Recombinant protein)
- Western blot analysis of N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL SARS-CoV-2 N recombinant monoclonal antibody, clone 85E9 (Cat # RAB01065).
- Enzyme-linked Immunoabsorbent Assay
- Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and SARS-CoV NP at 1 ug/mL. Purified SARS-CoV-2 N recombinant monoclonal antibody, clone 85E9 (Cat # RAB01065) was serially diluted 1:2 starting at 1 ug/mL. Rabbit monoclonal antibody 85E9 shows very strong and specific binding to both COVID-19 NP antigen and recognizes SARS-CoV NP antigen.
- Application Image
- Western Blot (Recombinant protein)
- enlarge
- Enzyme-linked Immunoabsorbent Assay
- enlarge
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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