Abnova/SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin)/1kit/RAB01062
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美元价：
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联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Recombinant rabbit monoclonal antibody raised against SARS-CoV-2 N.
- Immunogen:
- A synthetic peptide corresponding to C-terminus of SARS-CoV-2 N.
- Host:
- Rabbit
- Reactivity:
- SARS-CoV-2
- Specificity:
- Highly-specific to SARS-CoV-2 nucleoprotein; does not cross-react with nucleoprotein from SARS-CoV or other coronaviruses.
- Form:
- Liquid
- Conjugation:
- Biotin
- Preparation Method:
- 85C10 is derived from a Fab phage display library made from a rabbit immunized with synthetic peptide specific to the C-terminus of SARS-CoV-2 nucleocapsid protein. Libraries were selected on N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and Fab sup were screened on SARS-CoV-2 NP and SARS-CoV (2003) NP by ELISA. Positive clones were cloned into a bi-cistronic IgG vector and produced in HEK293 cells. The antibodies were purified from a Protein A column.
- Purification:
- Protein A purification
- Concentration:
- 0.5 mg/mL
- Isotype:
- IgG1, kappa
- Recommend Usage:
- ELISAThe optimal working dilution should be determined by the end user.
- Storage Buffer:
- In 1X PBS (0.02% sodium azide).
- Storage Instruction:
- Store at 4°C.
- Note:
- For highest sensitivity, we recommend pairing unconjugated clone 75G5a (Cat # RAB01053), immobilized on the ELISA plate to capture COVID-19 NP antigen, with SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin) (Cat # RAB01062) for detection using high-sensitivity streptavidin-HRP (Pierce # 21130). Other unconjugated and biotinylated anti-NP clones are provided as alternatives and also show high sensitivity and specificity. 75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), 84D7 (Cat # RAB01057), 85C1 (Cat # RAB01059), 85C10 (Cat # RAB01061), 85B4 (Cat # RAB01063), and 85E9 (Cat # RAB01065) can be directly coated to the ELISA wells as a capture antibody and the other antibody (e.g. biotinylated) [75G5a (Cat # RAB01054), 84C4a (Cat # RAB01056), 84D7 (Cat # RAB01058), 85C1 (Cat # RAB01060), 85C10 (Cat # RAB01062), 85B4 (Cat # RAB01064), and 85E9 (Cat # RAB01066)] can be used as a detecting antibody with StreptAvidin-HRP.This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Datasheet:
Download
- Applications
- Enzyme-linked Immunoabsorbent Assay
- Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) at 1 ug/mL. SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin) (Cat # RAB01062) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.
- Sandwich ELISA
- A sandwich ELISA was performed using SARS-CoV-2 N recombinant monoclonal antibody, clone 75G5a (Cat # RAB01053) as a capture antibody and SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin) (Cat # RAB01062) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 20 ng/mL. Recombinant rabbit monoclonal antibodies 75G5a and 85C10-biotin detected COVID-19 NP antigen at very high sensitivity as low as 19.5 pg/mL (1.9 pg).
- Application Image
- Enzyme-linked Immunoabsorbent Assay
- enlarge
- Sandwich ELISA
- enlarge
品牌介绍
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