Abnova/ALK分裂CISH探头/1kit/CS0001
市场价：
¥0.00
美元价：
0.00
产品分类：
电转仪
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- ALK Split CISH Probe is designed for the qualitative detection of translocations involving the human ALK gene at 2p23.2 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
- Recommend Usage:
- The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
- Supplied Product:
- Reagent Provided:This Probe is composed of:1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 2p23.2* (chr2:29,460,144-29,681,581) proximal to the ALK breakpoint region.2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 2p23.2* (chr2:29,174,204-29,383,335) distal to the ALK breakpoint region.3. Formamide based hybridization buffer.*according to Human Genome Assembly GRCh37/hg19
- Storage Instruction:
- Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
- Note:
- The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Interpretation of results:Using the CISH Implementation Kit 2 (Cat # KA5366), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (proximal to the ALK breakpoint region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (distal to the ALK breakpoint region).Normal situation: In interphases of normal cells or cells without a translocation involving the ALK gene region, two red/green fusion signals appear.Aberrant situation: One ALK gene region affected by a translocation is indicated by one separate green signal and one separate red signal. EML4-ALK inversion with deletion of 5"-ALK sequences is indicated by one or multiple isolated red signals.Overlapping signals may appear as brown signals. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.
- Probe Position:
- Regulatory Status:
- For research use only (RUO)
- Interpretation of Result:
- Datasheet:
Download
- Applications
- Chromogenic In Situ Hybridization (FFPE Tissue)
- Lung carcinoma tissue section with translocation affecting the 2p23.2 locus as indicated by one red/green fusion (non-rearranged) signal, one red signal, and one separate green signal.
- Application Image
- Chromogenic In Situ Hybridization (FFPE Tissue)
- enlarge
- Gene Information
- Entrez GeneID:
- 238
- Gene Name:
- ALK
- Gene Alias:
- CD246,Ki-1,TFG/ALK
- Gene Description:
- anaplastic lymphoma receptor tyrosine kinase
- Omim ID:
- 105590
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3" half of ALK, derived from chromosome 2, is fused to the 5" portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeq
- Other Designations:
- ALK tyrosine kinase receptor,CD246 antigen,anaplastic lymphoma kinase (Ki-1),anaplastic lymphoma kinase Ki-1
- Related Disease
- Adenocarcinoma
- Carcinoma, Non-Small-Cell Lung
- Genetic Predisposition to Disease
- Kidney Failure, Chronic
- Lung Neoplasms
- Multiple Sclerosis
- Schizophrenia
- Schizophrenia
- Tobacco Use Disorder
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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