当前位置：
首页
>
产品中心 >
Genetic_mutations >
Abnova/NME1 monoclonal antibody (M02), clone 1D7/1kit/H00004830-M02
Abnova/NME1 monoclonal antibody (M02), clone 1D7/1kit/H00004830-M02
商品编号：
H00004830-M02
品牌：
abnovadx
市场价：
¥0.00
美元价：
0.00
产品分类：
基因突变
公司分类：
Genetic_mutations
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant NME1.
- Immunogen:
- NME1 (NP_000260, 43 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- ASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
- Storage Buffer:
- In 1x PBS, pH 7.4
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- MSDS:
Download
- Interspecies Antigen Sequence:
- Mouse (94%); Rat (95%)
- Datasheet:
- Download
- Publication Reference
- 1.
- A transcriptome-based protein network that identifies new therapeutic targets in colorectal cancer.Durand S, Trillet K, Uguen A, Saint-Pierre A, Le Jossic-Corcos C, Corcos L.BMC Genomics. 2017 Sep 30;18(1):758.
- 2.
- Identification of Antigenic Proteins Associated with Trichloroethylene-Induced Autoimmune Disease by Serological Proteome Analysis.Liu J, Xing X, Huang H, Jiang Y, He H, Xu X, Yuan J, Zhou L, Yang L, Zhuang Z.Toxicol Appl Pharmacol. 2009 Nov 1;240(3):393-400. Epub 2009 Aug 6.
- Applications
- Western Blot (Cell lysate)
- NME1 monoclonal antibody (M02), clone 1D7. Western Blot analysis of NME1 expression in HeLa(Cat # L013V1 ).
- Protocol Download
- Western Blot (Cell lysate)
- NME1 monoclonal antibody (M02), clone 1D7. Western Blot analysis of NME1 expression in different cell lines.
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.
- Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml]
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of NME1 transfected lysate using anti-NME1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NME1 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.
- Protocol Download
- ELISA
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunofluorescence
- enlarge
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Immunoprecipitation
- enlarge
- Sandwich ELISA (Recombinant protein)
- enlarge
- ELISA
- Gene Information
- Entrez GeneID:
- 4830
- GeneBank Accession#:
- NM_000269
- Protein Accession#:
- NP_000260
- Gene Name:
- NME1
- Gene Alias:
- AWD,GAAD,NB,NBS,NDPK-A,NDPKA,NM23,NM23-H1
- Gene Description:
- non-metastatic cells 1, protein (NM23A) expressed in
- Omim ID:
- 156490, 256700
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of "A" (encoded by this gene) and "B" (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
- Other Designations:
- NDP kinase A,OTTHUMP00000174772
- Gene Pathway
- Metabolic pathways
- Purine metabolism
- Pyrimidine metabolism
- Related Disease
- Breast cancer
- Breast Neoplasms
- Carcinoma, Non-Small-Cell Lung
- Endometrial Neoplasms
- Genetic Predisposition to Disease
- Lung Neoplasms
- Neoplasm Metastasis
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
联络我们
