Abnova/MAPKAP激酶2多克隆抗体/1kit/PAB10263
市场价：
¥0.00
美元价：
0.00
产品分类：
克隆试剂盒
公司分类：
Cloning_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MAPKAP Kinase 2.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 310-325 of rabbit MAPKAP Kinase 2.
- Sequence:
- MTSALATMRVDYEQIK
- Host:
- Rabbit
- Reactivity:
- Chicken, Clawed frog, Hamster, Human, Mouse, Rabbit, Rat
- Specificity:
- This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues and extracts.
- Form:
- Liquid
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:15000)Western Blot (1:2000)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Datasheet:
Download
- Publication Reference
- 1.
- Retaining of the assembly capability of vimentin phosphorylated by mitogen-activated protein kinase-activated protein kinase-2.Cheng TJ, Tseng YF, Chang WM, Chang MD, Lai YK.J Cell Biochem. 2003 Jun 1;89(3):589-602.
- 2.
- Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs.Rousseau S, Morrice N, Peggie M, Campbell DG, Gaestel M, Cohen P.EMBO J. 2002 Dec 2;21(23):6505-14.
- 3.
- MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis.Kotlyarov A, Neininger A, Schubert C, Eckert R, Birchmeier C, Volk HD, Gaestel M.Nat Cell Biol. 1999 Jun;1(2):94-7.
- Applications
- Western Blot (Cell lysate)
- Affinity purified MAPKAP Kinase 2 polyclonal antibody (Cat # PAB10263) detects MAPKAP Kinase 2 in unstimulated human HeLa whole cell lysate by western blot.MAPKAP Kinase 2 polyclonal antibody used at a 1 : 2000 dilution to detect 20 ug of whole cell lysate containing MAPKAP Kinase 2.This antibody detects a single 44 KDa protein as indicated in crude extracts prepared from either unstimulatedor TNFalpha stimulated human HeLa cell lysates.A 4-20% gradient gel was used to separate the protein by SDS-PAGE.The protein was transferred to nitrocellulose using standard methods.After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.LICOR"s Odyssey® Infrared Imaging System was used to scan and process the image.
- Enzyme-linked Immunoabsorbent Assay
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Enzyme-linked Immunoabsorbent Assay
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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