Abnova/AKT1 (phospho S473) polyclonal antibody/1kit/PAB10323
市场价：
¥0.00
美元价：
0.00
产品分类：
克隆试剂盒
公司分类：
Cloning_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S473 of human AKT1.
- Host:
- Rabbit
- Reactivity:
- Chicken, Human, Mouse, Rat
- Specificity:
- This antibody is specific to phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT.
- Form:
- Liquid
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:15000-1:60000)Western Blot (1:200-1:1000)Immunohistochemistry (1:100-1:500)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Datasheet:
Download
- Publication Reference
- 1.
- PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?Lawlor MA, Alessi DR.J Cell Sci. 2001 Aug;114(Pt 16):2903-10.
- Applications
- Western Blot (Cell lysate)
- Immunoblotting of AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used at a 1:200 dilution to detect phosphorylated AKT1 by Western blot. A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT1 kinase was used.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used at a 1 : 100 dilution to detect AKT1 by immunohistochemistry of formalin-fixed and paraffin embedded in (A) human breast tumor tissue, (B) a higher magnification and (C) normal human breast tissue of the breast tumor.The staining is much stronger than the weak basal level of phosphorylation in normal breast.AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used with no pretreatment of tissue.Signal was developed using Dako"s Techmate streptavidin-biotin reagents.
- Immunofluorescence
- Immunofluorescence microscopy.Confocal image using AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) at a 1 : 40 dilution to detect phosphorylated AKT1 (green) in rat cardiomyocytes infected with adenovirus expressing with wild-type AKT1 in conjunction with a texas-red conjugated phalloidin (red) to label filamentous actin.
- Enzyme-linked Immunoabsorbent Assay
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Immunofluorescence
- enlarge
- Enzyme-linked Immunoabsorbent Assay
- Gene Information
- Entrez GeneID:
- 207
- Protein Accession#:
- P31749;AAH00479
- Gene Name:
- AKT1
- Gene Alias:
- AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
- Gene Description:
- v-akt murine thymoma viral oncogene homolog 1
- Omim ID:
- 164730, 181500
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
- Other Designations:
- RAC-alpha serine/threonine-protein kinase,murine thymoma viral (v-akt) oncogene homolog-1,protein kinase B,rac protein kinase alpha
- Interactome
- Gene Pathway
- Acute myeloid leukemia
- Adipocytokine signaling pathway
- Apoptosis
- B cell receptor signaling pathway
- Chemokine signaling pathway
- Chronic myeloid leukemia
- Colorectal cancer
- Endometrial cancer
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
- Fc gamma R-mediated phagocytosis
- Focal adhesion
- Glioma
- Insulin signaling pathway
- Jak-STAT signaling pathway
- MAPK signaling pathway
- Melanoma
- mTOR signaling pathway
- Neurotrophin signaling pathway
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prostate cancer
- Renal cell carcinoma
- Small cell lung cancer
- T cell receptor signaling pathway
- Tight junction
- Toll-like receptor signaling pathway
- VEGF signaling pathway
- Related Disease
- Adenocarcinoma
- Adenocarcinoma, Papillary
- Alzheimer Disease
- Alzheimer disease
- Amphetamine-Related Disorders
- Atherosclerosis
- Basal Ganglia Diseases
- Bipolar Disorder
- Breast Neoplasms
- Calcinosis
- Carcinoma
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Papillary
- Carcinoma, Papillary, Follicular
- Cardiovascular Diseases
- Cognition
- Colonic Neoplasms
- Colorectal Neoplasms
- Coronary Artery Disease
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
联络我们
