Abnova/ATG12多克隆抗体/1kit/PAB11358
市场价：
¥0.00
美元价：
0.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against recombinant ATG12.
- Immunogen:
- Recombinant protein corresponding to Saccharomyces cerevisiae ATG12.
- Host:
- Rabbit
- Reactivity:
- Yeast
- Form:
- Lyophilized
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:1000-1:5000)Western Blot (1:2000)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.Aliquot after reconstitution to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Datasheet:
Download
- Publication Reference
- 1.
- Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate.Mizushima N, Kuma A, Kobayashi Y, Yamamoto A, Matsubae M, Takao T, Natsume T, Ohsumi Y, Yoshimori T.J Cell Sci. 2003 May 1;116(Pt 9):1679-88.
- 2.
- Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast.Kuma A, Mizushima N, Ishihara N, Ohsumi Y.J Biol Chem. 2002 May 24;277(21):18619-25. Epub 2002 Mar 15.
- 3.
- The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation.Suzuki K, Kirisako T, Kamada Y, Mizushima N, Noda T, Ohsumi Y.EMBO J. 2001 Nov 1;20(21):5971-81.
- Applications
- Western Blot (Recombinant protein)
- Immunoblot of APG12 fusion protein.ATG12 polyclonal antibody (Cat # PAB11358) generated by immunization with recombinant yeast ATG12 was tested by immunoblot against yeast lysates expressing the ATG12-GFP fusion protein and other UBL fusion proteins.All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.Panel A shows total protein staining using ponceau.Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX.Panel C shows specific reaction with ATG12 using a 1:2,000 dilution of ATG12 polyclonal antibody (Cat # PAB11358) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX.All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incu
- Enzyme-linked Immunoabsorbent Assay
- Application Image
- Western Blot (Recombinant protein)
- enlarge
- Enzyme-linked Immunoabsorbent Assay
- Gene Information
- Entrez GeneID:
- 852518
- Gene Name:
- ATG12
- Gene Alias:
- APG12
- Gene Description:
- Atg12p
- Gene Ontology:
- Hyperlink
- Other Designations:
- Ubiquitin-like modifier, conjugated via an isopeptide bond to a lysine residue of Atg5p by the E1 enzyme, Atg7p, and the E2 enzyme, Atg10p, a step that is essential for autophagy
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
联络我们
