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Abnova/5-methylcytosine (5-mC) monoclonal antibody, clone RM231/1kit/MAB15115
Abnova/5-methylcytosine (5-mC) monoclonal antibody, clone RM231/1kit/MAB15115
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产品分类：
克隆试剂盒
公司分类：
Cloning_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Rabbit monoclonal antibody raised against 5-methylcytosine (5-mC).
- Immunogen:
- 5-methylcytosine conjugated with BSA.
- Host:
- Rabbit
- Specificity:
- This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
- Form:
- Liquid
- Purification:
- Protein A affinity purification
- Isotype:
- IgG
- Recommend Usage:
- Dot Blot (0.5-2 ug/mL)ELISA (0.1-1 ug/mL)Immunocytochemistry (0.5-2 ug/mL)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)Methylated DNA Immunoprecipitation (0.2-2 ug/mL)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
- Storage Instruction:
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Datasheet:
Download
- Publication Reference
- 1.
- Discovery of a new predominant cytosine DNA modification that is linked to gene expression in malaria parasites.Hammam E, Ananda G, Sinha A, Scheidig-Benatar C, Bohec M, Preiser PR, Dedon PC, Scherf A, Vembar SS.Nucleic Acids Res. 2020 Jan 10;48(1):184-199. doi: 10.1093/nar/gkz1093.
- Applications
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunocytochemistry
- Immunocytochemistry staining of HeLa cells with 5-methylcytosine (5-mC) monoclonal antibody, clone RM231 (Cat # MAB15115) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green), and nuclei stained with DAPI (Blue).
- Enzyme-linked Immunoabsorbent Assay
- ELISA analysis of single stranded DNA with 5-methylcytosine (5-mC) monoclonal antibody, clone RM231 (Cat # MAB15115). The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of MAB15115 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
- Dot Blot
- Dot blot analysis of double stranded DNA with 5-methylcytosine (5-mC) monoclonal antibody, clone RM231 (Cat # MAB15115). The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with MAB15115.
- Methylated DNA Immunoprecipitation
- Methylated DNA Immunoprecipitation (MeDIP) analysis of 5-methylcytosine (5-mC) monoclonal antibody, clone RM231 (Cat # MAB15115) at a 2:1 DNA:Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1 ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.
- Application Image
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunocytochemistry
- enlarge
- Enzyme-linked Immunoabsorbent Assay
- enlarge
- Dot Blot
- enlarge
- Methylated DNA Immunoprecipitation
- enlarge
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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