Abnova/5-甲基胞嘧啶单克隆抗体，克隆5MC-CD/1kit/MAB6766
市场价：
¥0.00
美元价：
0.00
产品分类：
基因突变
公司分类：
Genetic_mutations
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Mouse monoclonal antibody raised against 5-Methylcytosine.
- Immunogen:
- 5-Methylcytosine conjugated with BSA.
- Host:
- Mouse
- Form:
- Liquid
- Isotype:
- IgM
- Recommend Usage:
- Immunocytochemistry (1:50-1:100)Western Blot (~1:1000)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS (50% glycerol).
- Storage Instruction:
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Datasheet:
Download
- Publication Reference
- 1.
- The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA.Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, Endo TA, Shinga J, Mizutani-Koseki Y, Toyoda T, Okamura K, Tajima S, Mitsuya K, Okano M, Koseki H.Nature. 2007 Dec 6;450(7171):908-12. Epub 2007 Nov 11.
- 2.
- A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes.Nishiyama R, Ito M, Yamaguchi Y, Koizumi N, Sano H.Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5925-30.
- 3.
- Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine.Sano H, Imokawa M, Sager R.Biochim Biophys Acta. 1988 Nov 10;951(1):157-65.
- Applications
- Immunocytochemistry
- Immunofluorescence
- Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamydomonas me-1 cells.Left : DAPI-stained cells.Middle : Cells stained with 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766) and FITC-conjugated 2nd antibody.Right : Merged image Chloroplast DNA is exclusively methylated in gamete cells.
- Immunofluorescence
- Detection of DNA methylation in mouse embryonic stem cells by immunofluorescence staining with the 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766). Intense 5-methylcytosine staining at pericentromeric regions was seen in the mitotic chromosome and interphase nuclei of ESCs.
- Application Image
- Immunocytochemistry
- Immunofluorescence
- enlarge
- Immunofluorescence
- enlarge
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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