Abnova/5-mC monoclonal antibody, clone b/1kit/MAB4952
市场价：
¥0.00
美元价：
0.00
产品分类：
克隆试剂盒
公司分类：
Cloning_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Mouse monoclonal antibody raised against 5-methyl Cytosine.
- Immunogen:
- Ovalbumine conjugated with 5-methyl Cytosine.
- Host:
- Mouse
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Isotype:
- IgG1
- Recommend Usage:
- ELISA (0.1-0.3 ug/mL)Dot Blot (1-2 ug/mL)Immunofluorescence (1:100)Flow Cytometry (1-2 ug/mL)Methylated DNA Immunoprecipitation (1-5 ug/IP)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS
- Storage Instruction:
- Aliquot and store at -80°C.Aliquot to avoid repeated freezing and thawing.
- Datasheet:
Download
- Applications
- Immunofluorescence
- Indirect immunofluorescence results obtained with 5-mC monoclonal antibody, clone b (Cat # MAB4952).A. HeLa cells were immunofluorescent labelled with 5-mC monoclonal antibody, clone b (Cat # MAB4952) is diluted 1 : 100 followed by a goat anti-mouse FITC conjugated antibody. Scale bar is 75 um.B. Enlarged picture corresponding to a region from the left panel (as indicated in A).C. Nuclei were DAPI stained to label specifically the DNA (same region as shown in the middle panel : B).
- Immunoprecipitation
- MeDIP assay was performed using fragmented genomic DNA from the MCF7 breast cancer cells, 5-mC monoclonal antibody, clone b (Cat # MAB4952) and optimized PCR primer sets for qPCR of the indicated regions.1 ug of antibody was used per IP experiment.The hypermethylated p14 INK4A intronic CpG island and Alu repeats are clearly recovered, whereas the non-methylated promoter-CpG island of the p14 INK4A gene is not (red bars).One IP negative control (no antibody added) was included in the assay, the background is extremely low (white bars).
- Enzyme-linked Immunoabsorbent Assay
- Dot Blot
- Methylated DNA Immunoprecipitation
- Flow Cytometry
- Application Image
- Immunofluorescence
- enlarge
- Immunoprecipitation
- enlarge
- Enzyme-linked Immunoabsorbent Assay
- Dot Blot
- Methylated DNA Immunoprecipitation
- Flow Cytometry
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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