Abnova/GAPDH单克隆抗体，克隆6C5/1kit/MAB2549
市场价：
¥0.00
美元价：
0.00
产品分类：
转录表达
公司分类：
Target_Expression
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Mouse monoclonal antibody raised against native GAPDH.
- Immunogen:
- Native purified rabbit muscle GAPDH (whole molecule).
- Host:
- Mouse
- Theoretical MW (kDa):
- 36
- Reactivity:
- Dog, Human, Mouse, Rabbit, Rat
- Specificity:
- This antibody recognizes GAPDH monomer (36 KDa) and the dimer form.
- Form:
- Liquid
- Isotype:
- IgG1
- Quality Control Testing:
- Antibody Reactive Against Native Purified Protein.
- Recommend Usage:
- ELISA (0.01-0.1 ug/mL)Flow CytometryImmunohistochemistryImmunoprecipitation (2-5 ug/mL)Western Blot (0.1-1 ug/mL)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS, pH 7.2 (Antibody Stabilizer).
- Storage Instruction:
- Store at 4°C for one month. For long term storage store at -20°C to -80°C.Aliquot to avoid repeated freezing and thawing.
- Datasheet:
Download
- Publication Reference
- 1.
- Loss of enhancer of zeste homologue 2 (EZH2) at tumor invasion front is correlated with higher aggressiveness in colorectal cancer cells.Böhm J, Muenzner JK, Caliskan A, Ndreshkjana B, Erlenbach-Wünsch K, Merkel S, Croner R, Rau TT, Geppert CI, Hartmann A, Roehe AV, Schneider-Stock R.J Cancer Res Clin Oncol. 2019 Sep;145(9):2227-2240. doi: 10.1007/s00432-019-02977-1. Epub 2019 Jul 17.
- 2.
- Sensitive, robust and automated protein analysis of cell differentiation and of primary human blood cells by intact cell MALDI mass spectrometry biotyping.Munteanu B, von Reitzenstein C, Hansch GM, Meyer B, Hopf C.Anal Bioanal Chem. 2012 Sep 6.
- Applications
- Western Blot (Tissue lysate)
- Tissue lysate from mouse muscle was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by GAPDH monoclonal antibody, clone 6C5 (Cat # MAB2549) at 1 : 500.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemical staining of human colon tissue stained with GAPDH monoclonal antibody, clone 6C5 (Cat # MAB2549) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.
- Immunoprecipitation
- Enzyme-linked Immunoabsorbent Assay
- Flow Cytometry
- Application Image
- Western Blot (Tissue lysate)
- enlarge
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Immunoprecipitation
- Enzyme-linked Immunoabsorbent Assay
- Flow Cytometry
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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