Abnova/N (SARS-CoV-2) Recombinant Protein/1kit/P6626
市场价：
¥0.00
美元价：
0.00
产品分类：
细菌抗原
公司分类：
Bacterial_antigen
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- SARS-CoV-2 N (QHD43423.2) full-length recombinant protein with His tag at C-terminal expressed in Escherichia coli.
- Host:
- Escherichia coli
- Theoretical MW (kDa):
- 47.08
- Specificity:
- No non-specific binding to normal human serum was identified so far.
- Form:
- Liquid
- Preparation Method:
- Escherichia coli expression system
- Purification:
- Ni column
- Purity:
- > 95% by SDS-PAGE.
- Quality Control Testing:
- DTT-reducing SDS-PAGE Result
- Recommend Usage:
- AntigenELISAWestern BlotThe optimal working dilution should be determined by the end user.
- Storage Buffer:
- In 20 mM Tris, pH 8.5.
- Storage Instruction:
- This protein is not stable at 4°C in liquid. For long term storage store at -20 to -80°C.Aliquot to avoid repeated freezing and thawing.
- Datasheet:
Download
- Applications
- Western Blot
- Antigen
- Enzyme-linked Immunoabsorbent Assay
- ELISA plate was coated by N protein, 100 uL/well, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hour at 37°C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2 M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.
- SDS-PAGE
- Application Image
- Western Blot
- Antigen
- Enzyme-linked Immunoabsorbent Assay
- enlarge
- SDS-PAGE
品牌介绍
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