Abnova/SARS-CoV N monoclonal antibody, clone 3861/1kit/MAB22373
市场价：
¥0.00
美元价：
0.00
产品分类：
克隆试剂盒
公司分类：
Cloning_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Mouse monoclonal antibody raised against recombinant SARS-CoV N.
- Immunogen:
- Recombinant protein corresponding to amino acids 1-49 of SARS-CoV Nucleoprotein.
- Host:
- Mouse
- Reactivity:
- SARS-CoV
- Specificity:
- Specific for the nucleoprotein of the SARS-CoV-2 (lower reactivity) and the SARS-CoV (higher reactivity). This antibody cross react with native SARS virus and with recombinant Covid-19 NP. They do not cross react with native MERS virus.
- Form:
- Liquid
- Purification:
- Protein A chromoatography or sequential differential precipitations
- Isotype:
- IgG1
- Recommend Usage:
- ELISAImmunofluorescenceWestern BlotThe optimal working dilution should be determined by the end user.
- Storage Buffer:
- In 0.01 M PBS, pH 7.2 (0.09% sodium azide).
- Storage Instruction:
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Datasheet:
Download
- Applications
- Western Blot (Recombinant protein)
- Western Blot with human recombinant protein. Loading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV N monoclonal antibody, clone 3861 (Cat # MAB22373), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 1 ug/mL, Lane 2: 2 ug/mL.
- Immunofluorescence
- Enzyme-linked Immunoabsorbent Assay
- An ELISA was performed (A) using human SARS-CoV-2 Nucleocapsid recombinant protein as coating antigen and the SARS-CoV N monoclonal antibody, clone 3861 (Cat # MAB22373), as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 16 ng/mL to 1000 ng/mL. (B) using human SARS-CoV Nucleocapsid recombinant protein as coating antigen and the SARS-CoV N monoclonal antibody, clone 3861 (Cat # MAB22373), as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000 ng/mL.
- Application Image
- Western Blot (Recombinant protein)
- enlarge
- Immunofluorescence
- Enzyme-linked Immunoabsorbent Assay
- enlarge
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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