Abnova/SARS冠状病毒3CL蛋白酶多克隆抗体/1kit/PAB11368
市场价：
¥0.00
美元价：
0.00
产品分类：
双杂交系统
公司分类：
Two_Hybrid_Systems
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against full length recombinant SARS Coronavirus 3CL Protease.
- Immunogen:
- Recombinant protein corresponding to full length SARS Coronavirus 3CL Protease.
- Host:
- Rabbit
- Reactivity:
- SARS-CoV
- Specificity:
- This protein A purified antibody is directed against SARS Coronavirus 3CL Protease.
- Form:
- Lyophilized
- Purification:
- The product was purified from monospecific antiserum by protein A affinity purification.
- Isotype:
- IgG
- Recommend Usage:
- ELISA (1:10000-1:50000)ImmunofluorescenceWestern Blot (1:2000-1:10000)The optimal working dilution should be determined by the end user.
- Storage Buffer:
- Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
- Storage Instruction:
- Store at 4°C on dry atmosphere prior to restoration.After reconstitution with 0.1 mL of deionized water (or equivalent), for extended storage aliquot contents and freeze at -20°C or below.Aliquot to avoid repeated freezing and thawing.Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Datasheet:
Download
- Publication Reference
- 1.
- Expression and purification of SARS coronavirus proteins using SUMO-fusions.Zuo X, Mattern MR, Tan R, Li S, Hall J, Sterner DE, Shoo J, Tran H, Lim P, Sarafianos SG, Kazi L, Navas-Martin S, Weiss SR, Butt TR.Protein Expr Purif. 2005 Jul;42(1):100-10. Epub 2005 Feb 23.
- 2.
- Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli.Zuo X, Li S, Hall J, Mattern MR, Tran H, Shoo J, Tan R, Weiss SR, Butt TR.J Struct Funct Genomics. 2005;6(2-3):103-11.
- 3.
- SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins.Malakhov MP, Mattern MR, Malakhova OA, Drinker M, Weeks SD, Butt TR.J Struct Funct Genomics. 2004;5(1-2):75-86.
- Applications
- Western Blot (Recombinant protein)
- Western blot using SARS-CoV 3CL Protease polyclonal antibody (Cat # PAB11368) shows detection of a 34- KDa band corresponding to the protein.Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose.The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h.
- Immunofluorescence
- Enzyme-linked Immunoabsorbent Assay
- Application Image
- Western Blot (Recombinant protein)
- enlarge
- Immunofluorescence
- Enzyme-linked Immunoabsorbent Assay
- Gene Information
- Entrez GeneID:
- 1489680
- Protein Accession#:
- 29837498; P0C6U8
- Gene Name:
- orf1ab
- Gene Alias:
- -
- Gene Description:
- orf1ab polyprotein (pp1ab)
- Gene Ontology:
- Hyperlink
- Other Designations:
- orf1a polyprotein (pp1a)
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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