Abnova/RNA标记低易/1kit/R0002
市场价：
¥0.00
美元价：
0.00
产品分类：
链霉亲和素结合物
公司分类：
Streptavidin_conjugate
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- The RNA Marker Low Easy is supplied in a ready-to-use mixture of loading dye (containing formamide, EDTA sodium salt, bromphenol blue) and RNAs. It is prepared for denaturing polyacrylamide gel electrophoresis but not agarose gel elctrophoresis. The RNA Marker Low Easy has seven single-stranded RNAs, 20, 50, 100, 200, 300, 400 and 500 bases. The 20-base and 50-base RNA are synthesized by chemically (not phosphorylated), others are synthesized by in vitro transcription. In 5 ul of the RNA Marker Low Easy, each RNA amount is approximately 100 ng. It is useful for estimating RNA amount approximately. The RNA Marker Low Easy can be visualized by UV light after ethidium bromide staining.
- Quality Control Testing:
- After 18 hr incubation of the RNA Marker Low Easy at 37°C, no visible degradation of the marker is observed in 5 % polyacrylamide / 8M urea gel electrophoresis.
- Recommend Usage:
- 5 uL is recommended for loading to a well (0.1 ug of each RNA / 5 uL)
- Supplied Product:
- RNA Loading buffer PARNA Loading buffer PA is manufactured for denaturing polyacrylamide gel electrophoresis but not agarose gel elctrophoresis. The loading buffer has a composition of 80% formamide, 10 mM EDTA sodium salt (pH 8.0), 0.025% bromphenol blue. Store RNA Loading buffer PA at -80 °C. Repeated freeze/thaw cycles should be avoided. It is 1 × to 2 × solution. Use more than one volume of RNA solution.
- Storage Instruction:
- Store at -80 °C. Repeated freeze/thaw cycles should be avoided.
- Note:
- RNA is very sensitive to degradation by nucleases. To avoid damaging the RNA Marker Low Easy, use extreme care during manipulations to prevent nuclease contamination. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the product should be high grade and nuclease-free. To use, thaw the RNA Marker Low Easy on ice and keep it on ice while using. For heat denaturation, transfer aliquot of the RNA Marker Low Easy to another tube, then heat it . Avoid repeated heat denaturizing.Formamide is suspected to be harmful. It is irritate to the eyes and skin. Wear appropriate gloves and safety glasses. Put a lid tightly at the time of storage.
- Protocol:
Protocol Download
- MSDS:
- Download
- Datasheet:
- Download
- Applications
- Electrophoresis
- Application Image
- Electrophoresis
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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