Abnova/RNA标记低/1kit/R0001
市场价：
¥0.00
美元价：
0.00
产品分类：
免疫组化染色试剂盒
公司分类：
Immunostain_staining_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- The RNA Marker Low consists of seven single-stranded RNAs. The 20-base and 50-base RNA are synthesized by chemically (not phosphorylated). The 100, 200, 300, 400 and 500 bases are synthesized by in vitro transcription. The RNA Marker Low is suitable for determinating size of single-stranded RNAs in denaturing polyacrylamide gel electrophoresis. The concentration of each RNA (20-500 base) in the marker is approximately 0.1 ug/ul. It is useful for estimating of RNA amount. The RNA Marker Low can be visualized by ethidium bromide staining.
- Quality Control Testing:
- After 18 hr incubation of the RNA Marker Low at 37°C, no visible degradation of the marker is observed in 5 % polyacrylamide / 8M urea gel electrophoresis.
- Recommend Usage:
- 1 uL (0.7 ug) RNA marker low mix with 5 uL of gel loading buffer.
- Storage Buffer:
- In 10 mM Tris-HCl (pH 8.0) buffer containing 1 mM EDTA.
- Storage Instruction:
- Store at -80 °C. Repeated freeze/thaw cycles should be avoided.
- Note:
- RNA is very sensitive to degradation by nucleases. To avoid damaging the RNA Marker Low, use extreme care during manipulations to prevent nuclease contamination. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the marker should be high grade and nuclease-free. To use, thaw the RNA Marker Low on ice and keep it on ice while using.
- Protocol:
Protocol Download
- Datasheet:
- Download
- Publication Reference
- 1.
- Hepatitis C virus genomic RNA dimerization is mediated via a kissing complex intermediate.Shetty S, Kim S, Shimakami T, Lemon SM, Mihailescu MR.RNA. 2010 May;16(5):913-25. Epub 2010 Apr 1.
- 2.
- Identification of small RNAs in Mycobacterium tuberculosis.Arnvig KB, Young DB.Mol Microbiol. 2009 Aug;73(3):397-408. Epub 2009 Jun 22.
- Applications
- Electrophoresis
- Application Image
- Electrophoresis
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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