Abnova/RNA标记高易/1kit/R0004
市场价：
¥0.00
美元价：
0.00
产品分类：
反应底物
公司分类：
Reaction_substrate
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Specification
- Product Description:
- The RNA Marker High Easy is manufactured for easy measurement of RNA size on agarose gel electrophoresis. With RNA Marker High Easy, electrophorsis is possible on non-denaturing agarose gel as well as on denaturing agarose gel. To use RNA Marker High Easy, just mix RNA loading buffer AG with formaldehyde solution (not included in this kit), and added the mixture to RNA Marker High Easy or RNA sample, then heat to load into wells of agarose gel. The RNA loading buffer AG+ is a gel loading buffer prepared not only for denaturing agarose gel electrophoresis but also for non-contained denaturing agarose gel electrophoresis. RNA Marker High Easy contains nine single-stranded RNAs, 500, 1,000, 1,500, 2,000, 3,000, 4,000, 5,000 and 8,000 bases. Each RNA is 0.1 ug/ul approximately. It is useful for estimating RNA amount approximately. After electrophoresis, RNA bands on agarose gel can be visualized by UV light without further ethidium bromide staining.
- Quality Control Testing:
- After 18 hr incubation of RNA Marker High Easy at 37°C, no visible degradation of the marker is observed in formaldehyde-agarose (1%) gel electrophoresis.
- Supplied Product:
- RNA loading buffer B
- Storage Instruction:
- Store at -80 °C. Repeated freeze/thaw cycles should be avoided.
- Note:
- RNA is very sensitive to degradation by nuclease. To prevent nuclease contamination, extreme care is required for manipulation. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the marker should be high grade and nuclease-free. To use, thaw the RNA Marker High Easy on ice. Keep RNA Marker High Easy and RNA loading buffer B on ice while using. This kit can be used for non-denaturing agarose gel electrophoresis. Although adequate results to size RNAs were obtained as far as we tested, denaturing agarose gel electrophoresis may be required for a strict experiment. Formamide is suspected to be harmful. It is irritate to the eyes and skin. Ethidium bromide is a strong mutagen and susupected to be toxic. Wear appropriate gloves and safety glasses in using solutions and materials containing formamide and/or etidium bromide. Put a lid tightly at the time of storage. Formaldehyde is carcinogen and toxic. Avoid breathing the vapors. Wear appropriate gloves and safety glasses. Gel containing formaldehyde should be prepared and used in a chemical hood.
- Protocol:
Protocol Download
- Datasheet:
- Download
- Applications
- Electrophoresis
- Application Image
- Electrophoresis
品牌介绍
Abnova是世界上最大的抗体制造商。亚诺法的所有产品，重组蛋白和抗体都是利用最新的技术及由SPF等级动物实验室制造生产出来。我们的目标是生产人类基因组内每个基因至少要有一个相对应的抗体，可以提供给来自于学术机构、行业的伙伴和合作者做研究。亚诺法有许多多样化的单克隆及多克隆抗体，我们更进一步开发一系列的整合系统与相关应用包含配对抗体、ELISA试剂盒与荧光原位杂交(FISH)探针。这些工具对生物标记开发与转译研究都是必须的。亚诺法亦有针对糖尿病、传染疾病与癌症的体外检验试剂专案以及用于治疗败血症的全人类抗体药技专案，为全人类的健康努力。
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